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Interaction detection method:

Name:	animal immunization
pubmed	123456789 [primary-reference]
psi-par	PAR:1078 [identity]

Participant identification method:

Name:	predetermined: predetermined participant
pubmed	14755292 [primary-reference]
psi-par	PAR:0396 [identity]

author-list

Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA.

journal

J. Clin. Microbiol. (0095-1137)

publication year

1989

exp-modification

Female BALB/c

journal

Anal. Biochem. (0003-2697)

publication year

2003

exp-modification

The HSA was mixed with IgM and IgG.

Experiment #6

Name:

weiler-2003-2

Description:

A proteomics-based approach for monoclonal antibody characterization.

pubmed

14511687 [primary reference]

psi-par

PAR:1317 [exp scope]

Host Organism:

in vitro (taxid: -1)

Interaction detection method:

Name:	hybridoma generation
pubmed	14755292 [primary-reference]
psi-par	PAR:1105 [identity]

Participant identification method:

Name:	elisa: enzyme linked immunosorbent assay (go synonym: ELISA)
pubmed	14755292 [primary-reference]
psi-par	PAR:0411 [identity]
PMID	11906746 [primary-reference]

author-list

Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA.

journal

Anal. Biochem. (0003-2697)

publication year

2003

exp-modification

Splenocytes from immunized Balb/c mice were fused with the mouse myeloma cell line SP2/0 and subsequently cloned twice using limiting dilution cloning. Six putative anti-HSA clones were picked for further analysis. The cells were grown to 5x10E5 cells/mL in RPMI-1640 containing 10% fetal bovine serum. In some cases, cells were washed with serum-free RPMI and grown at 2.5x105 cells/mL in serum-free hybridoma media (Gibco Invitrogen Corp., Burlington, ON, Canada) until death (approximately 2 weeks). The supernatants were collected and used as a source of antibodies.

Experiment #25

Name:

weiler-2003-3

Description:

A proteomics-based approach for monoclonal antibody characterization.

pubmed

14511687 [primary reference]

psi-par

PAR:1317 [exp scope]

Host Organism:

in vitro (taxid: -1)

Interaction detection method:

Name:	unknown method
pubmed	14755292 [primary-reference]
psi-par	PAR:1176 [identity]

Participant identification method:

Name:	predetermined: predetermined participant
pubmed	14755292 [primary-reference]
psi-par	PAR:0396 [identity]

author-list

Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA.

journal

Anal. Biochem. (0003-2697)

publication year

2003

experimental kit

The antibodies were isotyped using the Isotyping Monoclonal Antibodies Kit from Amersham Biosciences (Baie dUrfe, PQ, Canada).

hybridoma

Splenocytes from immunized Balb/c mice were fused with the mouse myeloma cell line SP2/0.

Experiment #44

Name:

weiler-2003-4

Description:

A proteomics-based approach for monoclonal antibody characterization.

pubmed

14511687 [primary reference]

psi-par

PAR:113 [exp scope]

Host Organism:

in vitro (taxid: -1)

Interaction detection method:

Name:	elisa: enzyme linked immunosorbent assay (go synonym: ELISA)
pubmed	11906746 [primary-reference]
pubmed	14755292 [primary-reference]
psi-par	PAR:0411 [identity]
PMID	11906746 [primary-reference]

Participant identification method:

Name:	antibody detection: identification by antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:0421 [identity]

exp-modification

The second/detector antibody was an alkaline phosphatase conjugate of rabbit anti-mouse IgG (Sigma-Aldrich)

author-list

Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA.

journal

Anal. Biochem. (0003-2697)

publication year

2003

Experiment #120

Name:

weiler-2003-5

Description:

A proteomics-based approach for monoclonal antibody characterization.

pubmed

14511687 [primary reference]

psi-par

PAR:113 [exp scope]

Host Organism:

in vitro (taxid: -1)

Interaction detection method:

Name:	western blot (go synonym: Immuno blot)
pubmed	14755292 [primary-reference]
psi-par	PAR:0113 [identity]

Participant identification method:

Name:	antibody detection: identification by antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:0421 [identity]

exp-modification

The second/detector antibody was an alkaline phosphatase conjugate of rabbit anti-mouse IgG (Sigma-Aldrich)

author-list

Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA.

journal

Anal. Biochem. (0003-2697)

publication year

2003

equipment

MultiScreen apparatus (Bio-Rad, Mississauga, ON, Canada)

Experiment #139

Name:

weiler-2003-6

Description:

A proteomics-based approach for monoclonal antibody characterization.

pubmed

14511687 [primary reference]

psi-par

PAR:1128 [exp scope]

Host Organism:

in vitro (taxid: -1)

Interaction detection method:

Name:	pull down
pubmed	14755292 [primary-reference]
psi-par	PAR:0096 [identity]

Participant identification method:

Name:	fingerprinting: peptide massfingerprinting
pubmed	11752590 [method reference]
pubmed	10967324 [primary-reference]
pubmed	11805826 [see-also]
psi-par	PAR:0082 [identity]

author-list

Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA.

journal

Anal. Biochem. (0003-2697)

publication year

2003

exp-modification

Culture supernatants of the clones were used to arm Sepharose beads coupled to goat anti-mouse IgG.

software

The identities of the captured proteins were determined with Profound searches (Proteometrics Canada).

equipment

Knexus automation client was used to analyse spectra.

Experiment #244

Name:

weiler-2003-7

Description:

Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay.

pubmed

14511687 [primary reference]

psi-par

PAR:113 [exp scope]

Host Organism:

in vitro (taxid: -1)

Interaction detection method:

Name:	elisa: enzyme linked immunosorbent assay (go synonym: ELISA)
pubmed	11906746 [primary-reference]
pubmed	14755292 [primary-reference]
psi-par	PAR:0411 [identity]
PMID	11906746 [primary-reference]

Participant identification method:

Name:	antibody detection: identification by antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:0421 [identity]

author-list

Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA.

journal

Anal. Biochem. (0003-2697)

publication year

2003

exp-modification

An alkaline phosphatase conjugate of rabbit anti-mouse IgG (Sigma-Aldrich), 1/2000 in blocking buffer, was used as a secondary antibody.

InteractorList

Interactor #4

Name:

q56g89_human: Serum albumin

uniprotkb

Q56G89 [identity]

interpro

interpro

interpro

ensembl

ENSG00000163631

Interactor Type:

Name:	protein
so	SO:0000358 [identity]
pubmed	14755292 [primary-reference]
psi-par	PAR:0326 [identity]

Organism:

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Sequence:

MKWVTFISLL FLFSSAYSRG VFRRDAHKSE VAHRFKDLGE ENFKALVLIA FAQYLQQCPF EDHVKLVNEV TEFAKTCVAD ESAENCDKSL HTLFGDKLCT VATLRETYGE MADCCAKQEP ERNECFLQHK DDNPNLPRLV RPEVDVMCTA FHDNEETFLK KYLYEIARRH PYFYAPELLF FAKRYKAAFT ECCQAADKAA CLLPKLDELR DEGKASSAKQ GLKCASLQKF GERAFKAWAV ARLSQRFPKA EFAEVSKLVT DLTKVHTECC HGDLLECADD RADLAKYICE NQDSISSKLK ECCEKPLLEK SHCIAEVEND EMPADLPSLA ADFVGSKDVC KNYAEAKDVF LGMFLYEYAR RHPDYSVVLL LRLAKTYETT LEKCCAAADP HECYAKVFDE FKPLVEEPQN LIKQNCELFE QLGEYKFQNA LLVRYTKKVP QVSTPTLVEV SRNLGKVGSK CCKHPEAKRM PCAEDCLSVF LNQLCVLHEK TPVSDRVTKC CTESLVNGRP CFSALEVDET YVPKEFNAET FTFHADICTL SEKERQIKKQ TALVELVKHK PKATKEQLKA VMDDFAAFVE KCCKADDKET CFAEEGKKLV AASQAALGL

Interactor #8

Name:

hybridoma_6g11: Hybridoma of Balb/c mice splenocytes and SP2/0

Interactor Type:

Name:	ab-produci hybridoma: antibody-producing hybridoma
pubmed	14755292 [primary-reference]
psi-par	PAR:1008 [identity]

Organism:

Interactor #11

Name:

hybridoma_7b3: Hybridoma of Balb/c mice splenocytes and SP2/0

Interactor Type:

Name:	ab-produci hybridoma: antibody-producing hybridoma
pubmed	14755292 [primary-reference]
psi-par	PAR:1008 [identity]

Organism:

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Interactor #14

Name:

hybridoma_10c9: Hybridoma of Balb/c mice splenocytes and SP2/0

Interactor Type:

Name:	ab-produci hybridoma: antibody-producing hybridoma
pubmed	14755292 [primary-reference]
psi-par	PAR:1008 [identity]

Organism:

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Interactor #17

Name:

hybridoma_11g9: Hybridoma of Balb/c mice splenocytes and SP2/0

Interactor Type:

Name:	ab-produci hybridoma: antibody-producing hybridoma
pubmed	14755292 [primary-reference]
psi-par	PAR:1008 [identity]

Organism:

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Interactor #20

Name:

hybridoma_13b4: Hybridoma of Balb/c mice splenocytes and SP2/0

Interactor Type:

Name:	ab-produci hybridoma: antibody-producing hybridoma
pubmed	14755292 [primary-reference]
psi-par	PAR:1008 [identity]

Organism:

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Interactor #23

Name:

hybridoma_15b10: Hybridoma of Balb/c mice splenocytes and SP2/0

Interactor Type:

Name:	ab-produci hybridoma: antibody-producing hybridoma
pubmed	14755292 [primary-reference]
psi-par	PAR:1008 [identity]

Organism:

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Interactor #27

Name:

anti-hsa_mab_6g11: 6G11 mAb produced from immunization

Interactor Type:

Name:	mab: monoclonal antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:1051 [identity]

Organism:

antibody isoform

IgG1

Interactor #30

Name:

anti-hsa_mab_7b3: 7b3 mAb produced from immunization

Interactor Type:

Name:	mab: monoclonal antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:1051 [identity]

Organism:

antibody isoform

IgG1

Interactor #33

Name:

anti-hsa_mab_10c9: 10c9 mAb produced from immunization

Interactor Type:

Name:	mab: monoclonal antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:1051 [identity]

Organism:

antibody isoform

IgG1

Interactor #36

Name:

anti-hsa_mab_11g9: 11G9 mAb produced from immunization

Interactor Type:

Name:	mab: monoclonal antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:1051 [identity]

Organism:

antibody isoform

IgG1

Interactor #39

Name:

anti-hsa_mab_13b4: 13B4 mAb produced from immunization

Interactor Type:

Name:	mab: monoclonal antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:1051 [identity]

Organism:

antibody isoform

IgG1

Interactor #42

Name:

anti-hsa_mab_15b10: 15B10 mAb produced from immunization

Interactor Type:

Name:	mab: monoclonal antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:1051 [identity]

Organism:

antibody isoform

IgG1

Interactor #49

Name:

mab_chessie6: A monoclonal antibody called chessie6 in ATCC

pubmed

8068416 [see-also]

Interactor Type:

Name:	mab: monoclonal antibody
pubmed	14755292 [primary-reference]
psi-par	PAR:1051 [identity]

Organism:

Interactor #53

Name:

medium

Interactor Type:

Name:	growth medium
pubmed	14755292 [primary-reference]
psi-par	PAR:1156 [identity]

Organism:

in vitro (taxid: -1)

Interactor #57

Name:

mouse_serum: mouse serum diluted 500-fold in PBS

Interactor Type:

Name:	serum
pubmed	14755292 [primary-reference]
psi-par	PAR:1158 [identity]

Organism:

Interactor #144

Name:

g3bp1_human: Ras GTPase-activating protein-binding protein 1 (gene name: G3BP1) (gene name synonym: G3BP)

uniprotkb

Q13283 [identity]

interpro

interpro

interpro

ensembl

ENSG00000145907

Interactor Type:

Name:	protein
so	SO:0000358 [identity]
pubmed	14755292 [primary-reference]
psi-par	PAR:0326 [identity]

Organism:

k562_cell_line: K562 cell line

Sequence:

MVMEKPSPLL VGREFVRQYY TLLNQAPDML HRFYGKNSSY VHGGLDSNGK PADAVYGQKE IHRKVMSQNF TNCHTKIRHV DAHATLNDGV VVQVMGLLSN NNQALRRFMQ TFVLAPEGSV ANKFYVHNDI FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAPEDAQKS SSPAPADIAQ TVQEDLRTFS WASVTSKNLP PSGAVPVTGI PPHVVKVPAS QPRPESKPES QIPPQRPQRD QRVREQRINI PPQRGPRPIR EAGEQGDIEP RRMVRHPDSH QLFIGNLPHE VDKSELKDFF QSYGNVVELR INSGGKLPNF GFVVFDDSEP VQKVLSNRPI MFRGEVRLNV EEKKTRAARE GDRRDNRLRG PGGPRGGLGG GMRGPPRGGM VQKPGFGVGR GLAPRQ

Interactor #146

Name:

k562_cell_lysate: cell lysate from K562

Interactor Type:

Name:	cell lysate
pubmed	14755292 [primary-reference]
psi-par	PAR:1155 [identity]

Organism:

supplier

ATCC, Rockville, MD

Interactor #152

Name:

synovial_fluid: synovial fluid

Interactor Type:

Name:	synovial fluid
pubmed	14755292 [primary-reference]
psi-par	PAR:1153 [identity]

Organism:

Interactor #156

Name:

albu_bovin: Serum albumin precursor (gene name: ALB)

uniprotkb

P02769 [identity]

uniprotkb

P04277 [secondary-ac]

uniprotkb

O02787 [secondary-ac]

uniprotkb

Q3SZR2 [secondary-ac]

uniprotkb

A5PJX3 [secondary-ac]

interpro

interpro

interpro

ensembl

ENSBTAG00000017121

Interactor Type:

Name:	protein
so	SO:0000358 [identity]
pubmed	14755292 [primary-reference]
psi-par	PAR:0326 [identity]

Organism:

bovin: Bos taurus

Sequence:

MKWVTFISLL LLFSSAYSRG VFRRDTHKSE IAHRFKDLGE EHFKGLVLIA FSQYLQQCPF DEHVKLVNEL TEFAKTCVAD ESHAGCEKSL HTLFGDELCK VASLRETYGD MADCCEKQEP ERNECFLSHK DDSPDLPKLK PDPNTLCDEF KADEKKFWGK YLYEIARRHP YFYAPELLYY ANKYNGVFQE CCQAEDKGAC LLPKIETMRE KVLASSARQR LRCASIQKFG ERALKAWSVA RLSQKFPKAE FVEVTKLVTD LTKVHKECCH GDLLECADDR ADLAKYICDN QDTISSKLKE CCDKPLLEKS HCIAEVEKDA IPENLPPLTA DFAEDKDVCK NYQEAKDAFL GSFLYEYSRR HPEYAVSVLL RLAKEYEATL EECCAKDDPH ACYSTVFDKL KHLVDEPQNL IKQNCDQFEK LGEYGFQNAL IVRYTRKVPQ VSTPTLVEVS RSLGKVGTRC CTKPESERMP CTEDYLSLIL NRLCVLHEKT PVSEKVTKCC TESLVNRRPC FSALTPDETY VPKAFDEKLF TFHADICTLP DTEKQIKKQT ALVELLKHKP KATEEQLKTV MENFVAFVDK CCAADDKEAC FAVEGPKLVV STQTALA

Interactor #158

Name:

myg_horse: Myoglobin (gene name: MB)

uniprotkb

P68082 [identity]

uniprotkb

P02188 [secondary-ac]

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

interpro

interpro

interpro

Interactor Type:

Name:	protein
so	SO:0000358 [identity]
pubmed	14755292 [primary-reference]
psi-par	PAR:0326 [identity]

Organism:

horse: Equus caballus

Sequence:

MGLSDGEWQQ VLNVWGKVEA DIAGHGQEVL IRLFTGHPET LEKFDKFKHL KTEAEMKASE DLKKHGTVVL TALGGILKKK GHHEAELKPL AQSHATKHKI PIKYLEFISD AIIHVLHSKH PGDFGADAQG AMTKALELFR NDIAAKYKEL GFQG

Interactor #161

Name:

trfe_human: Serotransferrin precursor (gene name: TF) (orf name: PRO1400)

uniprotkb

P02787 [identity]

uniprotkb

Q9NQB8 [secondary-ac]

uniprotkb

O43890 [secondary-ac]

uniprotkb

Q9UHV0 [secondary-ac]

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

rcsb pdb

interpro

interpro

ensembl

ENSG00000091513

Interactor Type:

Name:	protein
so	SO:0000358 [identity]
pubmed	14755292 [primary-reference]
psi-par	PAR:0326 [identity]

Organism:

Sequence:

MRLAVGALLV CAVLGLCLAV PDKTVRWCAV SEHEATKCQS FRDHMKSVIP SDGPSVACVK KASYLDCIRA IAANEADAVT LDAGLVYDAY LAPNNLKPVV AEFYGSKEDP QTFYYAVAVV KKDSGFQMNQ LRGKKSCHTG LGRSAGWNIP IGLLYCDLPE PRKPLEKAVA NFFSGSCAPC ADGTDFPQLC QLCPGCGCST LNQYFGYSGA FKCLKDGAGD VAFVKHSTIF ENLANKADRD QYELLCLDNT RKPVDEYKDC HLAQVPSHTV VARSMGGKED LIWELLNQAQ EHFGKDKSKE FQLFSSPHGK DLLFKDSAHG FLKVPPRMDA KMYLGYEYVT AIRNLREGTC PEAPTDECKP VKWCALSHHE RLKCDEWSVN SVGKIECVSA ETTEDCIAKI MNGEADAMSL DGGFVYIAGK CGLVPVLAEN YNKSDNCEDT PEAGYFAVAV VKKSASDLTW DNLKGKKSCH TAVGRTAGWN IPMGLLYNKI NHCRFDEFFS EGCAPGSKKD SSLCKLCMGS GLNLCEPNNK EGYYGYTGAF RCLVEKGDVA FVKHQTVPQN TGGKNPDPWA KNLNEKDYEL LCLDGTRKPV EEYANCHLAR APNHAVVTRK DKEACVHKIL RQQQHLFGSN VTDCSGNFCL FRSETKDLLF RDDTVCLAKL HDRNTYEKYL GEEYVKAVGN LRKCSTSSLL EACTFRRP

Interactor #171

Name:

hpt_human: Haptoglobin precursor (gene name: HP)

uniprotkb

P00738 [identity]

uniprotkb

Q0VAC4 [secondary-ac]

uniprotkb

Q6LBY9 [secondary-ac]

uniprotkb

P00737 [secondary-ac]

uniprotkb

Q2PP15 [secondary-ac]

uniprotkb

Q3B7J0 [secondary-ac]

interpro

interpro

interpro

interpro

ensembl

ENSG00000197711

Interactor Type:

Name:	protein
so	SO:0000358 [identity]
pubmed	14755292 [primary-reference]
psi-par	PAR:0326 [identity]

Organism:

Sequence:

MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ KTIAEN

InteractionList

Interaction #1

Name:

immunization_w_hsa: production of anti-HSA mAbs

Experiments:

weiler-2003-1

Participant #3

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	immunogen
pubmed	14755292 [primary-reference]
psi-par	PAR:1190 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	library screening (go synonym: library selection) (go synonym: library panning) (go synonym: library selection) (go synonym: library panning)
pubmed	14755292 [primary-reference]
psi-par	PAR:1309 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #5

Name:

mab_6g11_hybridoma: Production of anti-HSA hybridoma to be used for mAb generation.

intact

EBI-35131 (1) [preceding interaction]

Experiments:

weiler-2003-2

Participant #7

hybridoma_6g11

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	intermediate product
pubmed	14755292 [primary-reference]
psi-par	PAR:1126 [identity]

Host Organism:

in vitro (taxid: -1)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #9

Name:

mab_7b3_hybridoma: Production of anti-HSA hybridoma to be used for mAb generation.

intact

EBI-35131 (1) [preceding interaction]

Experiments:

weiler-2003-2

Participant #10

hybridoma_7b3

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	intermediate product
pubmed	14755292 [primary-reference]
psi-par	PAR:1126 [identity]

Host Organism:

in vitro (taxid: -1)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #12

Name:

mab_10c9_hybridoma: Production of anti-HSA hybridoma to be used for mAb generation.

intact

EBI-35131 (1) [preceding interaction]

Experiments:

weiler-2003-2

Participant #13

hybridoma_10c9

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	intermediate product
pubmed	14755292 [primary-reference]
psi-par	PAR:1126 [identity]

Host Organism:

in vitro (taxid: -1)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #15

Name:

mab_11g9_hybridoma: Production of anti-HSA hybridoma to be used for mAb generation.

intact

EBI-35131 (1) [preceding interaction]

Experiments:

weiler-2003-2

Participant #16

hybridoma_11g9

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	intermediate product
pubmed	14755292 [primary-reference]
psi-par	PAR:1126 [identity]

Host Organism:

in vitro (taxid: -1)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #18

Name:

mab_13b4_hybridoma: Production of anti-HSA hybridoma to be used for mAb generation.

intact

EBI-35131 (1) [preceding interaction]

Experiments:

weiler-2003-2

Participant #19

hybridoma_13b4

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	intermediate product
pubmed	14755292 [primary-reference]
psi-par	PAR:1126 [identity]

Host Organism:

in vitro (taxid: -1)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #21

Name:

mab_15b10_hybridoma: Production of anti-HSA hybridoma to be used for mAb generation.

intact

EBI-35131 (1) [preceding interaction]

Experiments:

weiler-2003-2

Participant #22

hybridoma_15b10

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	intermediate product
pubmed	14755292 [primary-reference]
psi-par	PAR:1126 [identity]

Host Organism:

in vitro (taxid: -1)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #24

Name:

mab_6g11_isolation: Isolation of the mAb 6g11 from hybridoma

intact

EBI-35173 (2) [preceding interaction]

Experiments:

weiler-2003-3

Participant #26

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	generation product
pubmed	14755292 [primary-reference]
psi-par	PAR:1154 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #28

Name:

mab_7b3_isolation: Isolation of the mAb 7b3 from hybridoma

intact

EBI-36580 (2) [preceding interaction]

Experiments:

weiler-2003-3

Participant #29

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	generation product
pubmed	14755292 [primary-reference]
psi-par	PAR:1154 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #31

Name:

mab_10c9_isolation: Isolation of the mAb 10c9 from hybridoma

intact

EBI-36582 (2) [preceding interaction]

Experiments:

weiler-2003-3

Participant #32

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	generation product
pubmed	14755292 [primary-reference]
psi-par	PAR:1154 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #34

Name:

mab_11g9_isolation: Isolation of the mAb 11g9 from hybridoma

intact

EBI-36584 (2) [preceding interaction]

Experiments:

weiler-2003-3

Participant #35

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	generation product
pubmed	14755292 [primary-reference]
psi-par	PAR:1154 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #37

Name:

mab_13b4_isolation: Isolation of the mAb 13b4 from hybridoma

intact

EBI-36586 (2) [preceding interaction]

Experiments:

weiler-2003-3

Participant #38

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	generation product
pubmed	14755292 [primary-reference]
psi-par	PAR:1154 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #40

Name:

mab_15b10_isolation: Isolation of the mAb 15b10 from hybridoma

intact

EBI-36588 (2) [preceding interaction]

Experiments:

weiler-2003-3

Participant #41

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	generation product
pubmed	14755292 [primary-reference]
psi-par	PAR:1154 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

Interaction #43

Name:

6g11-hsa_elisa: Assessment of the binding of mAb 6g11 and human serum albumin in ELISA.

Experiments:

weiler-2003-4

Participant #45

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

5 x 10 ⁰ (μL/mL)

Participant #46

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Parameters

volume:

100 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #47

Name:

6g11-chessie6_neg: Assessment of the binding of mAb 6g11 to an irrelevant antibody "Chessie 6" (negative control).

Experiments:

weiler-2003-4

Participant #48

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]
Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

10 x 10 ⁰ (μL)

Participant #50

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #51

Name:

6g11-medium_neg: Assessment of the binding of mAb 6g11 to medium (negative control).

Experiments:

weiler-2003-4

Participant #52

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]
Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Participant #54

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #55

Name:

6g11-serum_neg: Assessment of the binding of mAb 6g11 to mouse serum (negative control).

Experiments:

weiler-2003-4

Participant #56

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]
Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #58

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #59

Name:

7b3-hsa_elisa: Assessment of the binding of mAb 7b3 and human serum albumin in ELISA.

Experiments:

weiler-2003-4

Participant #60

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

5 x 10 ⁰ (μL/mL)

Participant #61

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Parameters

volume:

100 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #62

Name:

10c9-hsa_elisa: Assessment of the binding of mAb 10c9 and human serum albumin in ELISA.

Experiments:

weiler-2003-4

Participant #63

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Parameters

volume:

100 x 10 ⁰ (μL)

Participant #64

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

5 x 10 ⁰ (μL/mL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #65

Name:

11g9-hsa_elisa: Assessment of the binding of mAb 11g9 and human serum albumin in ELISA.

Experiments:

weiler-2003-4

Participant #66

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

5 x 10 ⁰ (μL/mL)

Participant #67

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Parameters

volume:

100 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #68

Name:

13b4-hsa_elisa: Assessment of the binding of mAb 13b4 and human serum albumin in ELISA.

Experiments:

weiler-2003-4

Participant #69

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Parameters

volume:

100 x 10 ⁰ (μL)

Participant #70

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

5 x 10 ⁰ (μL/mL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #71

Name:

15b10-hsa_elisa: Assessment of the binding of mAb 15b10 and human serum albumin in ELISA.

Experiments:

weiler-2003-4

Participant #72

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

5 x 10 ⁰ (μL/mL)

Participant #73

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Parameters

volume:

100 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #74

Name:

7b3-medium_neg: Assessment of the binding of mAb 7b3 to medium (negative control).

Experiments:

weiler-2003-4

Participant #75

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Participant #76

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #77

Name:

10c9-medium_neg: Assessment of the binding of mAb 10c9 to medium (negative control).

Experiments:

weiler-2003-4

Participant #78

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Participant #79

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #80

Name:

11g9-medium_neg: Assessment of the binding of mAb 11g9 to medium (negative control).

Experiments:

weiler-2003-4

Participant #81

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Participant #82

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #83

Name:

13b4-medium_neg: Assessment of the binding of mAb 13b4 to medium (negative control).

Experiments:

weiler-2003-4

Participant #84

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Participant #85

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #86

Name:

15b10-medium_neg: Assessment of the binding of mAb 15b10 to medium (negative control).

Experiments:

weiler-2003-4

Participant #87

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Participant #88

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #89

Name:

7b3-serum_neg: Assessment of the binding of mAb 7b3 to mouse serum (negative control).

Experiments:

weiler-2003-4

Participant #90

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

Participant #91

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #92

Name:

10c9-serum_neg: Assessment of the binding of mAb 10c9 to mouse serum (negative control).

Experiments:

weiler-2003-4

Participant #93

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

Participant #94

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #95

Name:

13b4-serum_neg: Assessment of the binding of mAb 13b4 to mouse serum (negative control).

Experiments:

weiler-2003-4

Participant #96

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

Participant #97

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #98

Name:

15b10-serum_neg: Assessment of the binding of mAb 15b10 to mouse serum (negative control).

Experiments:

weiler-2003-4

Participant #99

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

Participant #100

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #101

Name:

7b3-chessie6_neg: Assessment of the binding of mAb 7b3 to an irrelevant antibody "Chessie 6" (negative control).

Experiments:

weiler-2003-4

Participant #102

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

10 x 10 ⁰ (μL)

Participant #103

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #104

Name:

10c9-chessie6_neg: Assessment of the binding of mAb 10c9 to an irrelevant antibody "Chessie 6" (negative control).

Experiments:

weiler-2003-4

Participant #105

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

10 x 10 ⁰ (μL)

Participant #106

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #107

Name:

11g9-chessie6_neg: Assessment of the binding of mAb 11g9 to an irrelevant antibody "Chessie 6" (negative control).

Experiments:

weiler-2003-4

Participant #108

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

10 x 10 ⁰ (μL)

Participant #109

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #110

Name:

13b4-chessie6_neg: Assessment of the binding of mAb 13b4 to an irrelevant antibody "Chessie 6" (negative control).

Experiments:

weiler-2003-4

Participant #111

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

10 x 10 ⁰ (μL)

Participant #112

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #113

Name:

15b10-chessie6_neg: Assessment of the binding of mAb 15b10 to an irrelevant antibody "Chessie 6" (negative control).

Experiments:

weiler-2003-4

Participant #114

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

10 x 10 ⁰ (μL)

Participant #115

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #116

Name:

11g9-serum_neg: Assessment of the binding of mAb 11g9 to serum (negative control).

Experiments:

weiler-2003-4

Participant #117

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]
Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

Participant #118

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #119

Name:

6g11-hsa_wb: Western Blot of anti-HSA mAb 6g11

Experiments:

weiler-2003-5

Participant #121

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

volume:

0.6 x 10 ⁰ (μL)

Participant #122

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #123

Name:

7b3-hsa_wb_neg: Western Blot of anti-HSA mAb 7b3

Experiments:

weiler-2003-5

Participant #124

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #125

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

volume:

0.6 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #126

Name:

10c9-hsa_wb: Western Blot of anti-HSA mAb 10c9

Experiments:

weiler-2003-5

Participant #127

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

volume:

0.6 x 10 ⁰ (μL)

Participant #128

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #129

Name:

11g9-hsa_wb_neg: Western Blot of anti-HSA mAb 11g9

Experiments:

weiler-2003-5

Participant #130

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

volume:

0.6 x 10 ⁰ (μL)

Participant #131

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

negative

Interaction #132

Name:

13b4-hsa_wb: Western Blot of anti-HSA mAb 13b4

Experiments:

weiler-2003-5

Participant #133

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

volume:

0.6 x 10 ⁰ (μL)

Participant #134

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #135

Name:

15b10-hsa_wb: Western Blot of anti-HSA mAb 15b10

Experiments:

weiler-2003-5

Participant #136

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #137

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

volume:

0.6 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 1

Interaction #138

Name:

6g11-mix: Cross-reactivity assessment of anti-HSA mAb 6g11 in an artificial protein mix

Experiments:

weiler-2003-5

Participant #140

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (μL)

Participant #141

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

Interaction #142

Name:

6g11-lysate: Cross-reactivity assessment of anti-HSA mAb 6g11 in a cell lysate

Experiments:

weiler-2003-6

Participant #143

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	positive control
pubmed	14755292 [primary-reference]
psi-par	PAR:1144 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #145

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

in vitro (taxid: -1)

Participant #147

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #148

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 3, Figure 1

results comment

The antibody could selectively capture human serum albumin from the cell lysate.

Interaction #149

Name:

6g11-synovial_fluid: Cross-reactivity assessment of anti-HSA mAb 6g11 in synovial fluid

Experiments:

weiler-2003-6

Participant #150

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #151

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

Participant #153

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Figure 1, Figure 3

results comment

The antibody could selectively capture human serum albumin from synovial fluid.

Interaction #154

Name:

6g11-mix_neg: Cross-reactivity assessment of anti-HSA mAb 6g11 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #155

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #157

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #159

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #160

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

negative

Interaction #162

Name:

7b3-mix: Cross-reactivity assessment of anti-HSA mAb 7b3 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #163

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #164

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

Interaction #165

Name:

10c9-mix: Cross-reactivity assessment of anti-HSA mAb 10c9 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #166

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #167

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

Interaction #168

Name:

11g9-mix: Cross-reactivity assessment of anti-HSA mAb 11g9 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #169

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #170

hpt_human

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (μL)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

Interaction #172

Name:

13b4-mix: Cross-reactivity assessment of anti-HSA mAb 13b4 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #173

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (μL)

Participant #174

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

Interaction #175

Name:

15b10-mix: Cross-reactivity assessment of anti-HSA mAb 15b10 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #176

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (μL)

Participant #177

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

Interaction #178

Name:

7b3-mix_neg: Cross-reactivity assessment of anti-HSA mAb 7b3 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #179

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #180

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #181

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #182

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

negative

Interaction #183

Name:

10c9-mix_neg: Cross-reactivity assessment of anti-HSA mAb 10c9 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #184

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #185

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #186

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #187

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

negative

Interaction #188

Name:

11g9-mix_neg: Cross-reactivity assessment of anti-HSA mAb 11g9 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #189

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #190

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #191

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #192

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

negative

Interaction #193

Name:

13b4-mix_neg: Cross-reactivity assessment of anti-HSA mAb 13b4 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #194

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #195

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #196

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #197

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

negative

Interaction #198

Name:

15b10-mix_neg: Cross-reactivity assessment of anti-HSA mAb 15b10 in an artificial protein mix

Experiments:

weiler-2003-6

Participant #199

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #200

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Participant #201

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #202

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	negative control
pubmed	14755292 [primary-reference]
psi-par	PAR:1119 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Parameters

concentration:

1.5 x 10 ⁰ (nmol)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 2, Figure 1

negative

Interaction #203

Name:

7b3-synovial_fluid: Cross-reactivity assessment of anti-HSA mAb 7b3 in synovial fluid

Experiments:

weiler-2003-6

Participant #204

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

Participant #205

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #206

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Figure 1, Figure 3

results comment

The antibody could selectively capture human serum albumin from synovial fluid.

Interaction #207

Name:

10c9-synovial_fluid-: Cross-reactivity assessment of anti-HSA mAb 10c9 in synovial fluid

Experiments:

weiler-2003-6

Participant #208

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

Participant #209

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #210

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Figure 1, Figure 3

results comment

The antibody could selectively capture human serum albumin from synovial fluid.

Interaction #211

Name:

11g9-synovial_fluid-: Cross-reactivity assessment of anti-HSA mAb 11g9 in synovial fluid

Experiments:

weiler-2003-6

Participant #212

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

Participant #213

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #214

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Figure 1, Figure 3

results comment

The antibody could selectively capture human serum albumin from synovial fluid.

Interaction #215

Name:

13b4-synovial_fluid: Cross-reactivity assessment of anti-HSA mAb 13b4 in synovial fluid

Experiments:

weiler-2003-6

Participant #216

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

Participant #217

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #218

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Figure 1, Figure 3

results comment

The antibody could selectively capture human serum albumin from synovial fluid.

Interaction #219

Name:

15b10-synovial_fluid: Cross-reactivity assessment of anti-HSA mAb 15b10 in synovial fluid

Experiments:

weiler-2003-6

Participant #220

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

Participant #221

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #222

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Figure 1, Figure 3

results comment

The antibody could selectively capture human serum albumin from synovial fluid.

Interaction #223

Name:

7b3-lysate: Cross-reactivity assessment of anti-HSA mAb 7b3 in a cell lysate

Experiments:

weiler-2003-6

Participant #224

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

in vitro (taxid: -1)

Participant #225

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	positive control
pubmed	14755292 [primary-reference]
psi-par	PAR:1144 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #226

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #227

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 3, Figure 1

results comment

The antibody could selectively capture human serum albumin from the cell lysate.

Interaction #228

Name:

10c9-lysate: Cross-reactivity assessment of anti-HSA mAb 10c9 in a cell lysate

Experiments:

weiler-2003-6

Participant #229

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

in vitro (taxid: -1)

Participant #230

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	positive control
pubmed	14755292 [primary-reference]
psi-par	PAR:1144 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #231

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #232

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 3, Figure 1

results comment

The antibody could selectively capture human serum albumin from the cell lysate.

Interaction #233

Name:

13b4-lysate: Cross-reactivity assessment of anti-HSA mAb 13b4 in a cell lysate

Experiments:

weiler-2003-6

Participant #234

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

in vitro (taxid: -1)

Participant #235

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	positive control
pubmed	14755292 [primary-reference]
psi-par	PAR:1144 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #236

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #237

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 3, Figure 1

results comment

The antibody could selectively capture human serum albumin from the cell lysate.

Interaction #238

Name:

15b10-lysate: Cross-reactivity assessment of anti-HSA mAb 15b10 in a cell lysate

Experiments:

weiler-2003-6

Participant #239

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	positive control
pubmed	14755292 [primary-reference]
psi-par	PAR:1144 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #240

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

in vitro (taxid: -1)

Participant #241

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

Participant #242

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	physical interaction (go synonym: aggregation)
pubmed	14755292 [primary-reference]
psi-par	PAR:0218 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Table 3, Figure 1

results comment

The antibody could selectively capture human serum albumin from the cell lysate.

Interaction #243

Name:

11g9_hsa_affinity: Test of 10c9-hsa and 11g9-haptoglobin binding

Experiments:

weiler-2003-7

Participant #245

hpt_human

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	neutral component
pubmed	14755292 [primary-reference]
psi-par	PAR:0497 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #246

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Participant #247

Biological Role:

Name:	par target: protein affinity reagent target
pubmed	14755292 [primary-reference]
psi-par	PAR:1120 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Interaction Type:

Name:	direct interaction
pubmed	14755292 [primary-reference]
psi-par	PAR:0407 [identity]

Modelled

false

Intra molecular

false

Negative

false

figure legend

Figure 4a

Interaction #248

Name:

11g9_haptog_affinity: Test of the mAb 11g9 affinity to haptoglobin

Experiments:

weiler-2003-7

Participant #249

hpt_human

Biological Role:

Name:	unspecified role
pubmed	14755292 [primary-reference]
psi-par	PAR:0499 [identity]

Experimental Role:

Name:	bait
pubmed	14755292 [primary-reference]
psi-par	PAR:0496 [identity]

Host Organism:

unknown: Unknown (taxid: -3)

Participant #250

mouse_sp20_hybridoma: Hybridoma of mouse splenocytes and SP2/0 myoloma cell line

Biological Role:

Name:	affinity reagent: protein affinity reagent (go synonym: par)
pubmed	14755292 [primary-reference]
psi-par	PAR:1117 [identity]

Experimental Role:

Name:	prey
pubmed	14755292 [primary-reference]
psi-par	PAR:0498 [identity]

Host Organism:

Participant #251