ebi European Bioinformatics Institute weiler-2003-1 A proteomics-based approach for monoclonal antibody characterization. mouse Mus musculus animal immunization animal immunization predetermined predetermined participant Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. J. Clin. Microbiol. (0095-1137) 1989 Female BALB/c Anal. Biochem. (0003-2697) 2003 The HSA was mixed with IgM and IgG. weiler-2003-2 A proteomics-based approach for monoclonal antibody characterization. in vitro hybridoma generation hybridoma generation elisa enzyme linked immunosorbent assay ELISA Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. Anal. Biochem. (0003-2697) 2003 Splenocytes from immunized Balb/c mice were fused with the mouse myeloma cell line SP2/0 and subsequently cloned twice using limiting dilution cloning. Six putative anti-HSA clones were picked for further analysis. The cells were grown to 5x10E5 cells/mL in RPMI-1640 containing 10% fetal bovine serum. In some cases, cells were washed with serum-free RPMI and grown at 2.5x105 cells/mL in serum-free hybridoma media (Gibco Invitrogen Corp., Burlington, ON, Canada) until death (approximately 2 weeks). The supernatants were collected and used as a source of antibodies. weiler-2003-3 A proteomics-based approach for monoclonal antibody characterization. in vitro mAb harvesting mAb harvesting from hybridoma predetermined predetermined participant Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. Anal. Biochem. (0003-2697) 2003 The antibodies were isotyped using the Isotyping Monoclonal Antibodies Kit from Amersham Biosciences (Baie dUrfe, PQ, Canada). Splenocytes from immunized Balb/c mice were fused with the mouse myeloma cell line SP2/0. weiler-2003-4 A proteomics-based approach for monoclonal antibody characterization. in vitro elisa enzyme linked immunosorbent assay ELISA antibody detection identification by antibody The second/detector antibody was an alkaline phosphatase conjugate of rabbit anti-mouse IgG (Sigma-Aldrich) Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. Anal. Biochem. (0003-2697) 2003 weiler-2003-5 A proteomics-based approach for monoclonal antibody characterization. in vitro western blot western blot Immuno blot antibody detection identification by antibody The second/detector antibody was an alkaline phosphatase conjugate of rabbit anti-mouse IgG (Sigma-Aldrich) Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. Anal. Biochem. (0003-2697) 2003 MultiScreen apparatus (Bio-Rad, Mississauga, ON, Canada) weiler-2003-6 A proteomics-based approach for monoclonal antibody characterization. in vitro pull down pull down fingerprinting peptide massfingerprinting Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. Anal. Biochem. (0003-2697) 2003 Culture supernatants of the clones were used to arm Sepharose beads coupled to goat anti-mouse IgG. The identities of the captured proteins were determined with Profound searches (Proteometrics Canada). Knexus automation client was used to analyse spectra. weiler-2003-7 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro elisa enzyme linked immunosorbent assay ELISA antibody detection identification by antibody Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. Anal. Biochem. (0003-2697) 2003 An alkaline phosphatase conjugate of rabbit anti-mouse IgG (Sigma-Aldrich), 1/2000 in blocking buffer, was used as a secondary antibody. q56g89_human Serum albumin protein protein human Homo sapiens MKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQGLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVGSKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDCLSVFLNQLCVLHEKTPVSDRVTKCCTESLVNGRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL hybridoma_6g11 Hybridoma of Balb/c mice splenocytes and SP2/0 ab-produci hybridoma antibody-producing hybridoma mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line hybridoma_7b3 Hybridoma of Balb/c mice splenocytes and SP2/0 ab-produci hybridoma antibody-producing hybridoma mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line hybridoma_10c9 Hybridoma of Balb/c mice splenocytes and SP2/0 ab-produci hybridoma antibody-producing hybridoma mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line hybridoma_11g9 Hybridoma of Balb/c mice splenocytes and SP2/0 ab-produci hybridoma antibody-producing hybridoma mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line hybridoma_13b4 Hybridoma of Balb/c mice splenocytes and SP2/0 ab-produci hybridoma antibody-producing hybridoma mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line hybridoma_15b10 Hybridoma of Balb/c mice splenocytes and SP2/0 ab-produci hybridoma antibody-producing hybridoma mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line anti-hsa_mab_6g11 6G11 mAb produced from immunization mab monoclonal antibody mouse Mus musculus IgG1 anti-hsa_mab_7b3 7b3 mAb produced from immunization mab monoclonal antibody mouse Mus musculus IgG1 anti-hsa_mab_10c9 10c9 mAb produced from immunization mab monoclonal antibody mouse Mus musculus IgG1 anti-hsa_mab_11g9 11G9 mAb produced from immunization mab monoclonal antibody mouse Mus musculus IgG1 anti-hsa_mab_13b4 13B4 mAb produced from immunization mab monoclonal antibody mouse Mus musculus IgG1 anti-hsa_mab_15b10 15B10 mAb produced from immunization mab monoclonal antibody mouse Mus musculus IgG1 mab_chessie6 A monoclonal antibody called chessie6 in ATCC mab monoclonal antibody mouse Mus musculus medium medium growth medium growth medium in vitro mouse_serum mouse serum diluted 500-fold in PBS serum serum mouse Mus musculus g3bp1_human Ras GTPase-activating protein-binding protein 1 G3BP1 G3BP protein protein human Homo sapiens MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ k562_cell_lysate cell lysate from K562 cell lysate cell lysate k562_cell_line K562 cell line ATCC, Rockville, MD synovial_fluid synovial fluid synovial fluid synovial fluid human Homo sapiens albu_bovin Serum albumin precursor ALB protein protein bovin Bos taurus MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA myg_horse Myoglobin MB protein protein horse Equus caballus MGLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASEDLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQG trfe_human Serotransferrin precursor TF PRO1400 protein protein human Homo sapiens MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP hpt_human Haptoglobin precursor HP protein protein human Homo sapiens MSALGAVIALLLWGQLFAVDSGNDVTDIADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNDKKQWINKAVGDKLPECEADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNNEKQWINKAVGDKLPECEAVCGKPKNPANPVQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN immunization_w_hsa production of anti-HSA mAbs 2 q56g89_human 4 unspecified role unspecified role immunogen immunogen unknown Unknown SIgma-Aldrich library screening library screening library selection library panning library selection library panning mab_6g11_hybridoma Production of anti-HSA hybridoma to be used for mAb generation. 6 hybridoma_6g11 8 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation mab_7b3_hybridoma Production of anti-HSA hybridoma to be used for mAb generation. 6 hybridoma_7b3 11 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation mab_10c9_hybridoma Production of anti-HSA hybridoma to be used for mAb generation. 6 hybridoma_10c9 14 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation mab_11g9_hybridoma Production of anti-HSA hybridoma to be used for mAb generation. 6 hybridoma_11g9 17 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation mab_13b4_hybridoma Production of anti-HSA hybridoma to be used for mAb generation. 6 hybridoma_13b4 20 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation mab_15b10_hybridoma Production of anti-HSA hybridoma to be used for mAb generation. 6 hybridoma_15b10 23 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation mab_6g11_isolation Isolation of the mAb 6g11 from hybridoma 25 anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par generation product generation product mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation mab_7b3_isolation Isolation of the mAb 7b3 from hybridoma 25 anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par generation product generation product mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation mab_10c9_isolation Isolation of the mAb 10c9 from hybridoma 25 anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par generation product generation product mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation mab_11g9_isolation Isolation of the mAb 11g9 from hybridoma 25 anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par generation product generation product mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation mab_13b4_isolation Isolation of the mAb 13b4 from hybridoma 25 anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par generation product generation product mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation mab_15b10_isolation Isolation of the mAb 15b10 from hybridoma 25 anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par generation product generation product mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation 6g11-hsa_elisa Assessment of the binding of mAb 6g11 and human serum albumin in ELISA. 44 q56g89_human 4 par target protein affinity reagent target bait bait unknown Unknown 44 anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line 44 direct interaction direct interaction Table 1 6g11-chessie6_neg Assessment of the binding of mAb 6g11 to an irrelevant antibody "Chessie 6" (negative control). 44 mab_chessie6 49 unspecified role unspecified role negative control negative control bait bait unknown Unknown 44 anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 1 6g11-medium_neg Assessment of the binding of mAb 6g11 to medium (negative control). 44 medium 53 unspecified role unspecified role negative control negative control bait bait anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 6g11-serum_neg Assessment of the binding of mAb 6g11 to mouse serum (negative control). 44 mouse_serum 57 unspecified role unspecified role negative control negative control bait bait mouse Mus musculus anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 7b3-hsa_elisa Assessment of the binding of mAb 7b3 and human serum albumin in ELISA. 44 q56g89_human 4 par target protein affinity reagent target bait bait unknown Unknown 44 anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line 44 direct interaction direct interaction Table 1 10c9-hsa_elisa Assessment of the binding of mAb 10c9 and human serum albumin in ELISA. 44 anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line 44 q56g89_human 4 par target protein affinity reagent target bait bait unknown Unknown 44 direct interaction direct interaction Table 1 11g9-hsa_elisa Assessment of the binding of mAb 11g9 and human serum albumin in ELISA. 44 q56g89_human 4 par target protein affinity reagent target bait bait unknown Unknown 44 anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line 44 direct interaction direct interaction Table 1 13b4-hsa_elisa Assessment of the binding of mAb 13b4 and human serum albumin in ELISA. 44 anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line 44 q56g89_human 4 par target protein affinity reagent target bait bait unknown Unknown 44 direct interaction direct interaction Table 1 15b10-hsa_elisa Assessment of the binding of mAb 15b10 and human serum albumin in ELISA. 44 q56g89_human 4 par target protein affinity reagent target bait bait unknown Unknown 44 anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line 44 direct interaction direct interaction Table 1 7b3-medium_neg Assessment of the binding of mAb 7b3 to medium (negative control). 44 medium 53 unspecified role unspecified role bait bait negative control negative control anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 10c9-medium_neg Assessment of the binding of mAb 10c9 to medium (negative control). 44 medium 53 unspecified role unspecified role bait bait negative control negative control anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 11g9-medium_neg Assessment of the binding of mAb 11g9 to medium (negative control). 44 medium 53 unspecified role unspecified role bait bait negative control negative control anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 13b4-medium_neg Assessment of the binding of mAb 13b4 to medium (negative control). 44 medium 53 unspecified role unspecified role bait bait negative control negative control anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 15b10-medium_neg Assessment of the binding of mAb 15b10 to medium (negative control). 44 medium 53 unspecified role unspecified role bait bait negative control negative control anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 7b3-serum_neg Assessment of the binding of mAb 7b3 to mouse serum (negative control). 44 mouse_serum 57 unspecified role unspecified role bait bait negative control negative control mouse Mus musculus anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 10c9-serum_neg Assessment of the binding of mAb 10c9 to mouse serum (negative control). 44 mouse_serum 57 unspecified role unspecified role bait bait negative control negative control mouse Mus musculus anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 13b4-serum_neg Assessment of the binding of mAb 13b4 to mouse serum (negative control). 44 mouse_serum 57 unspecified role unspecified role bait bait negative control negative control mouse Mus musculus anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 15b10-serum_neg Assessment of the binding of mAb 15b10 to mouse serum (negative control). 44 mouse_serum 57 unspecified role unspecified role bait bait negative control negative control mouse Mus musculus anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 7b3-chessie6_neg Assessment of the binding of mAb 7b3 to an irrelevant antibody "Chessie 6" (negative control). 44 mab_chessie6 49 unspecified role unspecified role bait bait negative control negative control unknown Unknown 44 anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 1 10c9-chessie6_neg Assessment of the binding of mAb 10c9 to an irrelevant antibody "Chessie 6" (negative control). 44 mab_chessie6 49 unspecified role unspecified role bait bait negative control negative control unknown Unknown 44 anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 1 11g9-chessie6_neg Assessment of the binding of mAb 11g9 to an irrelevant antibody "Chessie 6" (negative control). 44 mab_chessie6 49 unspecified role unspecified role bait bait negative control negative control unknown Unknown 44 anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 1 13b4-chessie6_neg Assessment of the binding of mAb 13b4 to an irrelevant antibody "Chessie 6" (negative control). 44 mab_chessie6 49 unspecified role unspecified role bait bait negative control negative control unknown Unknown 44 anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 1 15b10-chessie6_neg Assessment of the binding of mAb 15b10 to an irrelevant antibody "Chessie 6" (negative control). 44 mab_chessie6 49 unspecified role unspecified role bait bait negative control negative control unknown Unknown 44 anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 1 11g9-serum_neg Assessment of the binding of mAb 11g9 to serum (negative control). 44 mouse_serum 57 unspecified role unspecified role bait bait negative control negative control mouse Mus musculus anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation true Table 1 6g11-hsa_wb Western Blot of anti-HSA mAb 6g11 120 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 120 anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction Table 1 7b3-hsa_wb_neg Western Blot of anti-HSA mAb 7b3 120 anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 120 direct interaction direct interaction true Table 1 10c9-hsa_wb Western Blot of anti-HSA mAb 10c9 120 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 120 anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction Table 1 11g9-hsa_wb_neg Western Blot of anti-HSA mAb 11g9 120 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 120 anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 1 13b4-hsa_wb Western Blot of anti-HSA mAb 13b4 120 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 120 anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction Table 1 15b10-hsa_wb Western Blot of anti-HSA mAb 15b10 120 anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 120 direct interaction direct interaction Table 1 6g11-mix Cross-reactivity assessment of anti-HSA mAb 6g11 in an artificial protein mix 120 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 139 anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction Table 2, Figure 1 6g11-lysate Cross-reactivity assessment of anti-HSA mAb 6g11 in a cell lysate 139 g3bp1_human 144 unspecified role unspecified role positive control positive control unknown Unknown BD Transduction Laboratories (Mississauga, ON, Canada) k562_cell_lysate 146 unspecified role unspecified role neutral component neutral component in vitro q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation Table 3, Figure 1 The antibody could selectively capture human serum albumin from the cell lysate. 6g11-synovial_fluid Cross-reactivity assessment of anti-HSA mAb 6g11 in synovial fluid 139 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown synovial_fluid 152 unspecified role unspecified role neutral component neutral component human Homo sapiens anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation Figure 1, Figure 3 The antibody could selectively capture human serum albumin from synovial fluid. 6g11-mix_neg Cross-reactivity assessment of anti-HSA mAb 6g11 in an artificial protein mix 139 albu_bovin 156 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada myg_horse 158 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada anti-hsa_mab_6g11 27 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line trfe_human 161 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada direct interaction direct interaction true Table 2, Figure 1 7b3-mix Cross-reactivity assessment of anti-HSA mAb 7b3 in an artificial protein mix 139 anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 139 direct interaction direct interaction Table 2, Figure 1 10c9-mix Cross-reactivity assessment of anti-HSA mAb 10c9 in an artificial protein mix 139 anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 139 direct interaction direct interaction Table 2, Figure 1 11g9-mix Cross-reactivity assessment of anti-HSA mAb 11g9 in an artificial protein mix 139 anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line hpt_human 171 par target protein affinity reagent target prey prey unknown Unknown 139 direct interaction direct interaction Table 2, Figure 1 13b4-mix Cross-reactivity assessment of anti-HSA mAb 13b4 in an artificial protein mix 139 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 139 anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction Table 2, Figure 1 15b10-mix Cross-reactivity assessment of anti-HSA mAb 15b10 in an artificial protein mix 139 q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown 139 anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction Table 2, Figure 1 7b3-mix_neg Cross-reactivity assessment of anti-HSA mAb 7b3 in an artificial protein mix 139 myg_horse 158 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada albu_bovin 156 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada trfe_human 161 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line direct interaction direct interaction true Table 2, Figure 1 10c9-mix_neg Cross-reactivity assessment of anti-HSA mAb 10c9 in an artificial protein mix 139 myg_horse 158 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada albu_bovin 156 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line trfe_human 161 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada direct interaction direct interaction true Table 2, Figure 1 11g9-mix_neg Cross-reactivity assessment of anti-HSA mAb 11g9 in an artificial protein mix 139 albu_bovin 156 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada myg_horse 158 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line trfe_human 161 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada direct interaction direct interaction true Table 2, Figure 1 13b4-mix_neg Cross-reactivity assessment of anti-HSA mAb 13b4 in an artificial protein mix 139 albu_bovin 156 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada myg_horse 158 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line trfe_human 161 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada direct interaction direct interaction true Table 2, Figure 1 15b10-mix_neg Cross-reactivity assessment of anti-HSA mAb 15b10 in an artificial protein mix 139 albu_bovin 156 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada myg_horse 158 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line trfe_human 161 unspecified role unspecified role negative control negative control unknown Unknown 139 Sigma-Aldrich Canada, Oakville, ON, Canada direct interaction direct interaction true Table 2, Figure 1 7b3-synovial_fluid Cross-reactivity assessment of anti-HSA mAb 7b3 in synovial fluid 139 synovial_fluid 152 unspecified role unspecified role neutral component neutral component human Homo sapiens anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Figure 1, Figure 3 The antibody could selectively capture human serum albumin from synovial fluid. 10c9-synovial_fluid- Cross-reactivity assessment of anti-HSA mAb 10c9 in synovial fluid 139 synovial_fluid 152 unspecified role unspecified role neutral component neutral component human Homo sapiens anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Figure 1, Figure 3 The antibody could selectively capture human serum albumin from synovial fluid. 11g9-synovial_fluid- Cross-reactivity assessment of anti-HSA mAb 11g9 in synovial fluid 139 synovial_fluid 152 unspecified role unspecified role neutral component neutral component human Homo sapiens anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Figure 1, Figure 3 The antibody could selectively capture human serum albumin from synovial fluid. 13b4-synovial_fluid Cross-reactivity assessment of anti-HSA mAb 13b4 in synovial fluid 139 synovial_fluid 152 unspecified role unspecified role neutral component neutral component human Homo sapiens anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Figure 1, Figure 3 The antibody could selectively capture human serum albumin from synovial fluid. 15b10-synovial_fluid Cross-reactivity assessment of anti-HSA mAb 15b10 in synovial fluid 139 synovial_fluid 152 unspecified role unspecified role neutral component neutral component human Homo sapiens anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Figure 1, Figure 3 The antibody could selectively capture human serum albumin from synovial fluid. 7b3-lysate Cross-reactivity assessment of anti-HSA mAb 7b3 in a cell lysate 139 k562_cell_lysate 146 unspecified role unspecified role neutral component neutral component in vitro g3bp1_human 144 unspecified role unspecified role positive control positive control unknown Unknown BD Transduction Laboratories (Mississauga, ON, Canada) q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown anti-hsa_mab_7b3 30 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line physical interaction physical interaction aggregation Table 3, Figure 1 The antibody could selectively capture human serum albumin from the cell lysate. 10c9-lysate Cross-reactivity assessment of anti-HSA mAb 10c9 in a cell lysate 139 k562_cell_lysate 146 unspecified role unspecified role neutral component neutral component in vitro g3bp1_human 144 unspecified role unspecified role positive control positive control unknown Unknown BD Transduction Laboratories (Mississauga, ON, Canada) anti-hsa_mab_10c9 33 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Table 3, Figure 1 The antibody could selectively capture human serum albumin from the cell lysate. 13b4-lysate Cross-reactivity assessment of anti-HSA mAb 13b4 in a cell lysate 139 k562_cell_lysate 146 unspecified role unspecified role neutral component neutral component in vitro g3bp1_human 144 unspecified role unspecified role positive control positive control unknown Unknown BD Transduction Laboratories (Mississauga, ON, Canada) anti-hsa_mab_13b4 39 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Table 3, Figure 1 The antibody could selectively capture human serum albumin from the cell lysate. 15b10-lysate Cross-reactivity assessment of anti-HSA mAb 15b10 in a cell lysate 139 g3bp1_human 144 unspecified role unspecified role positive control positive control unknown Unknown BD Transduction Laboratories (Mississauga, ON, Canada) k562_cell_lysate 146 unspecified role unspecified role neutral component neutral component in vitro anti-hsa_mab_15b10 42 affinity reagent protein affinity reagent par bait bait mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target prey prey unknown Unknown physical interaction physical interaction aggregation Table 3, Figure 1 The antibody could selectively capture human serum albumin from the cell lysate. 11g9_hsa_affinity Test of 10c9-hsa and 11g9-haptoglobin binding 244 hpt_human 171 unspecified role unspecified role neutral component neutral component unknown Unknown anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target bait bait unknown Unknown direct interaction direct interaction Figure 4a 11g9_haptog_affinity Test of the mAb 11g9 affinity to haptoglobin 244 hpt_human 171 unspecified role unspecified role bait bait unknown Unknown anti-hsa_mab_11g9 36 affinity reagent protein affinity reagent par prey prey mouse_sp20_hybridoma Hybridoma of mouse splenocytes and SP2/0 myoloma cell line q56g89_human 4 par target protein affinity reagent target neutral component neutral component unknown Unknown direct interaction direct interaction true Figure 4b