ebi European Bioinformatics Institute burns-1989-1 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro solvent extraction liquid-liquid extraction partitioning elisa enzyme linked immunosorbent assay ELISA Burns JW., Welch SK., Nakata S., Estes MK. J. Clin. Microbiol. (0095-1137) 1989 Virus extracted from faecal samples using trifluorotrichloroethane. burns-1989-2 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro solution sedimentati cosedimentation in solution electron microscopy electron microscopy Electron cryomicroscopy Electron crystallography Electron cryomicroscopy Electron crystallography Burns JW., Welch SK., Nakata S., Estes MK. J. Clin. Microbiol. (0095-1137) 1989 CsCl gradient burns-1989-3 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. mouse Mus musculus animal immunization animal immunization predetermined predetermined participant Weiler T., Sauder P., Cheng K., Ens W., Standing K., Wilkins JA. J. Clin. Microbiol. (0095-1137) 1989 Female BALB/c mice were hyperimmunized with the J-1 virus. burns-1989-4 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro hybridoma generation hybridoma generation hybridization elisa enzyme linked immunosorbent assay ELISA Burns JW., Welch SK., Nakata S., Estes MK. J. Clin. Microbiol. (0095-1137) 1989 Splenocytes were harvested and fused with P3X63 Ag8.653 murine myeloma cells using polyethylene glycol 4000. The fusion mixture was plated in 96-well tissue culture plates. The viable hybrids were screened with an ELISA. Hybridoma culture supernatants were added to coated and blocked microtiter plates containing CsCI gradient-purified virus. Positive cultures were expanded and subcloned twice by limiting dilution, and ascites fluids were produced in pristane-primed BALB/c mice. burns-1989-5 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro chromatography chromatography technology column chromatography elisa enzyme linked immunosorbent assay ELISA Burns JW., Welch SK., Nakata S., Estes MK. J. Clin. Microbiol. (0095-1137) 1989 The isotype of each individual MAb was determined with the MonoAb-ID enzyme immunoassay isotyping kit (Zymed Laboratories, South San Francisco, Calif.). The Bakerbond ABx column was used to purify mAbs from ascites fluid. The hybridoma was produced from female BALB/c mice splenocytes fused with P3X63 Ag8.653 murine myeloma cells. burns-1989-6 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro elisa enzyme linked immunosorbent assay ELISA antibody detection identification by antibody Burns JW., Welch SK., Nakata S., Estes MK. J. Clin. Microbiol. (0095-1137) 1989 Rabbit hyperimmune serum to human group B rotavirus was utilised as the detector antibody. burns-1989-7 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro elisa enzyme linked immunosorbent assay ELISA antibody detection identification by antibody Burns JW., Welch SK., Nakata S., Estes MK. J. Clin. Microbiol. (0095-1137) 1989 rabbit hyperimmune serum to human group B rotavirus as the detector antibody The Titertek Multiskan plater reader (Flow Laboratories, Inc., McLean, Va.) burns-1989-8 Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. in vitro competition binding competition binding elisa enzyme linked immunosorbent assay ELISA Burns JW., Welch SK., Nakata S., Estes MK. J. Clin. Microbiol. (0095-1137) 1989 rabbit hyperimmune serum to human group B rotavirus as the detector antibody Positive reactivity was determined to be an A414 of at least 0.100 and a ratio of >2.0 compared with preimmune guinea pig serum or MAb PAblOl (anti-simian virus 40 T antigen). virus_stool_extract Human group B rotavirus extract virus virus human Homo sapiens antij1_b5c9 mAb against human group B rotavirus mab monoclonal antibody mouse Mus musculus IgG1 antij1_b5e4 mAb against human group B rotavirus mab monoclonal antibody mouse Mus musculus IgG1 antij1_b10g10 mAb against human group B rotavirus mab monoclonal antibody mouse Mus musculus IgG2a j1_group_b_rotavirus J1 group B rotavirus virus virus human_rotavirus_b Human rotavirus B b5e4_hybridoma Hybridoma of mice splenocytes and P3X63 ab-produci hybridoma antibody-producing hybridoma mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma b10g10_hybridoma Hybridoma of mice splenocytes and P3X63 ab-produci hybridoma antibody-producing hybridoma mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma b5c9_hybridoma Hybridoma of mice splenocytes and P3X63 ab-produci hybridoma antibody-producing hybridoma mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma j1_stool_extract Stool extract of J-1 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B f1_stool_extract Extract of F1 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B g1_stool_extract Stool extract of G-1 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B s2_stool_extract Extract of S2 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B s3_stool_extract Extract of S3 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B s4_stool_extract Extract of S4 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B s9_stool_extract Extract of S9 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B s14_stool_extract Extract of S14 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B s15_stool_extract Extract of S15 human group B rotavirus virus virus human_rotavirus_b Human rotavirus B 57_non-grpb_samples This interactor represent 57 samples lacking human group B rotaviruses virus virus human_rotavirus_a Human rotavirus A Texas Childrens Hospital, Houston b5c9_epitope_compari Epitope mapping of b5c9 (immobilized), b5e4 and b10g10 mAbs 2 virus_stool_extract 4 par target protein affinity reagent target prey prey in vitro antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma 2 2 antij1_b5e4 8 affinity reagent protein affinity reagent par competitor competitor <new synonym> mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma antij1_b10g10 10 affinity reagent protein affinity reagent par neutral component neutral component mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation The mAbs bind to the outer capsid of the virus. mAb B5E4 competes with B5C9 and thus their epitopes overlap. Figure 3a b5e4_epitope_compari Epitope mapping of b5e4 (immobilized), b5c9 and b10g10 mAbs 2 antij1_b5c9 6 affinity reagent protein affinity reagent par competitor competitor <new synonym> mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma antij1_b10g10 10 affinity reagent protein affinity reagent par competitor competitor <new synonym> mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma 2 2 virus_stool_extract 4 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation The mAbs bind to the outer capsid of the virus. mAb B5E4 competes with B5C9 and thus their epitopes overlap. Figure 3b b10g10_epitope_compa Epitope mapping of b10g10 (immobilized), b5e4 and b5c9 mAbs 2 antij1_b5c9 6 affinity reagent protein affinity reagent par neutral component neutral component mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma antij1_b5e4 8 affinity reagent protein affinity reagent par competitor competitor <new synonym> mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma 2 2 virus_stool_extract 4 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation The mAbs bind to the outer capsid of the virus. mAb B5E4 competes with B5C9 and thus their epitopes overlap. Figure 3c immunization_w_j1 production of a group B rotavirus mAb 22 j1_group_b_rotavirus 24 unspecified role unspecified role immunogen immunogen human Homo sapiens physical interaction physical interaction aggregation antij1_b5e4_hybridom Production of hybridoma generating anti-J1 mAb b5e4 26 b5e4_hybridoma 28 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation antij1_b10g10_hybrid Production of hybridoma producing anti-J1 mAb b10g10 26 b10g10_hybridoma 31 unspecified role unspecified role intermediate product intermediate product in vitro physical interaction physical interaction aggregation antij1_b5c9_hybridom Production of hybridoma producing anti-J1 mAb b5c9 26 b5c9_hybridoma 34 unspecified role unspecified role intermediate product intermediate product mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation j1virus_partitioning Purification of human group B rotavirus samples 36 j1_stool_extract 38 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation f1virus_partitioning Purification of human group B rotavirus samples 36 f1_stool_extract 41 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation g1virus_partitioning Purification of human group B rotavirus samples 36 g1_stool_extract 44 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation s2virus_partitioning Purification of human group B rotavirus samples 36 s2_stool_extract 47 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation s3virus_partitioning Purification of human group B rotavirus samples 36 s3_stool_extract 50 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation s4virus_partitioning Purification of human group B rotavirus samples 36 s4_stool_extract 53 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation s9virus_partitioning Purification of human group B rotavirus samples 36 s9_stool_extract 56 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation s14virus_partitionin Purification of human group B rotavirus samples 36 s14_stool_extract 59 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation s15virus_partitionin Purification of human group B rotavirus samples 36 s15_stool_extract 62 par target protein affinity reagent target generation product generation product human Homo sapiens physical interaction physical interaction aggregation j1virus_purification Separation of the human J-1 human group B rotavirus 64 j1_group_b_rotavirus 24 par target protein affinity reagent target generation product generation product in vitro physical interaction physical interaction aggregation b5c9_specificity_neg Specificity binding assay of a the mAb b5c9 against 57 samples of human group B rotavirus 67 57_non-grpb_samples 69 unspecified role unspecified role negative control negative control in vitro antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation true Table 2 Only the non-binding interactions were annotated because the specific identity of the binding species ("15 group B rotavirus samples") is unknown. b5e4_specificity_neg Specificity binding assay of a the mAb b5e4 against 57 samples of human group B rotavirus 67 57_non-grpb_samples 69 unspecified role unspecified role negative control negative control in vitro antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation Table 2 Only the non-binding interactions were annotated because the specific identity of the binding species ("15 group B rotavirus samples") is unknown. b10g10_specifici_neg Specificity binding assay of a the mAb b10g10 against 57 samples of human group B rotavirus 67 57_non-grpb_samples 69 unspecified role unspecified role negative control negative control in vitro antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation Table 2 Only the non-binding interactions were annotated because the specific identity of the binding species ("15 group B rotavirus samples") is unknown. mab_b5e4_isolation Purification of anti-J1 virus mAb b5e4 from ascite fluid 78 antij1_b5e4 8 affinity reagent protein affinity reagent par generation product generation product mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation mab_b10g10_isolation Purification of anti-J1 virus mAb b10g10 from ascite fluid 78 antij1_b10g10 10 affinity reagent protein affinity reagent par generation product generation product mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation mab_b5c9_isolation Purification of anti-J1 virus mAb b5c9 from ascite fluid 78 antij1_b5c9 6 affinity reagent protein affinity reagent par generation product generation product mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation b5c9-f1_binding Binding assay of a the mAb b5c9 against an extract of F1 human group B rotavirus 78 f1_stool_extract 41 par target protein affinity reagent target prey prey in vitro antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-j1_binding Binding assay of a the mAb b5c9 against an extract of J1 human group B rotavirus 85 j1_stool_extract 38 par target protein affinity reagent target prey prey positive control positive control in vitro antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-g1_binding Binding assay of a the mAb b5c9 against an extract of G1 human group B rotavirus 85 g1_stool_extract 44 par target protein affinity reagent target prey prey in vitro antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-s2_binding Binding assay of a the mAb b5c9 against an extract of S2 human group B rotavirus 85 antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s2_stool_extract 47 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-s3_binding Binding assay of a the mAb b5c9 against an extract of S3 human group B rotavirus 85 antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s3_stool_extract 50 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-s4_binding Binding assay of a the mAb b5c9 against an extract of S4 human group B rotavirus 85 antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s4_stool_extract 53 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-s9_binding Binding assay of a the mAb b5c9 against an extract of S9 human group B rotavirus 85 s9_stool_extract 56 par target protein affinity reagent target prey prey in vitro antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-s14_binding Binding assay of a the mAb b5c9 against an extract of S14 human group B rotavirus 85 s14_stool_extract 59 par target protein affinity reagent target prey prey in vitro antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5c9-s15_binding Binding assay of a the mAb b5c9 against an extract of S15 human group B rotavirus 85 antij1_b5c9 6 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s15_stool_extract 62 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-j1_binding Binding assay of a the mAb b5e4 against an extract of J1 human group B rotavirus 85 antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma j1_stool_extract 38 par target protein affinity reagent target positive control positive control prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-f1_binding Binding assay of a the mAb b5e4 against an extract of F1 human group B rotavirus 85 f1_stool_extract 41 par target protein affinity reagent target prey prey in vitro antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-g1_binding Binding assay of a the mAb b5e4 against an extract of G1 human group B rotavirus 85 g1_stool_extract 44 par target protein affinity reagent target prey prey in vitro antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-s2_binding Binding assay of a the mAb b5e4 against an extract of S2 human group B rotavirus 85 antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s2_stool_extract 47 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-s3_binding Binding assay of a the mAb b5e4 against an extract of S3 human group B rotavirus 85 antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s3_stool_extract 50 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-s4_binding Binding assay of a the mAb b5e4 against an extract of S4 human group B rotavirus 85 s4_stool_extract 53 par target protein affinity reagent target prey prey in vitro antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-s9_binding Binding assay of a the mAb b5e4 against an extract of S9 human group B rotavirus 85 antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s9_stool_extract 56 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-s14_binding Binding assay of a the mAb b5e4 against an extract of S14 human group B rotavirus 85 antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s14_stool_extract 59 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b5e4-s15_binding Binding assay of a the mAb b5e4 against an extract of S15 human group B rotavirus 85 s15_stool_extract 62 par target protein affinity reagent target prey prey in vitro antij1_b5e4 8 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-j1_binding Binding assay of a the mAb b10g10 against an extract of J1 human group B rotavirus 85 antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma j1_stool_extract 38 par target protein affinity reagent target positive control positive control prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-f1_binding Binding assay of a the mAb b10g10 against an extract of F1 human group B rotavirus 85 f1_stool_extract 41 par target protein affinity reagent target prey prey in vitro antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-g1_binding Binding assay of a the mAb b10g10 against an extract of G1 human group B rotavirus 85 g1_stool_extract 44 par target protein affinity reagent target prey prey in vitro antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-s2_binding Binding assay of a the mAb b10g10 against an extract of S2 human group B rotavirus 85 antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s2_stool_extract 47 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-s3_binding Binding assay of a the mAb b10g10 against an extract of S3 human group B rotavirus 85 s3_stool_extract 50 par target protein affinity reagent target prey prey in vitro antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-s4_binding Binding assay of a the mAb b10g10 against an extract of S4 human group B rotavirus 85 s4_stool_extract 53 par target protein affinity reagent target prey prey in vitro antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-s9_binding Binding assay of a the mAb b10g10 against an extract of S9 human group B rotavirus 85 s9_stool_extract 56 par target protein affinity reagent target prey prey in vitro antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-s14_binding Binding assay of a the mAb b10g10 against an extract of S14 human group B rotavirus 85 antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s14_stool_extract 59 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody. b10g10-s15_binding Binding assay of a the mAb b10g10 against an extract of S15 human group B rotavirus 85 antij1_b10g10 10 affinity reagent protein affinity reagent par bait bait mouse_p3x63_hybridom mouse splenocyte and P3X63 hybridoma s15_stool_extract 62 par target protein affinity reagent target prey prey in vitro physical interaction physical interaction aggregation 85 Table 1 The numeric readout is the absorbance value at 414 nm. The normalised readout was produced by dividing the numeric readout of the antibody with the A414 value obtained with preimmune guinea pig serum as the capture antibody.