Minimum Information About a Proteomics Experiment

The context-sensitive nature of transcriptome, metabolome and proteome data necessitates the capture of a richer set of metadata (data about the data) than is required for basic genetic sequence, where usually knowing the organism of origin will suffice. The use of paper citations as proxies for actual metadata hinders the reassessment of data sets, and obstructs non-standard searching (e.g. by the order that different liquid chromatography columns were coupled). The requirements of the various journals also differ, so important detail may be lacking in some cases, or presented in an esoteric fashion.

There is then a need for public repositories that contain information from whole proteomics experiments; making explicit both where samples came from, and how analyses of them were performed. It is therefore appropriate to attempt to define the minimum set of information about a proteomics experiment that would be required by such a repository.

Published components of MIAPE:

The 'MIAPE Principles' document:
The minimum information about a proteomics experiment (MIAPE), Nature Biotechnology 25, 887-893 (2007) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Reporting guidelines for Molecular Interactions experiments:
The minimum information required for reporting a molecular interaction experiment (MIMIx), Nature Biotechnology 25, 894-898 (2007) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Reporting guidelines for Mass Spectrometry experiments:
Guidelines for reporting the use of mass spectrometry in proteomics, Nature Biotechnology 26, 860-861 (2008) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Reporting guidelines for Mass Spectrometry Informatics experiments:
Guidelines for reporting the use of mass spectrometry informatics in proteomics, Nature Biotechnology 26, 862 (2008) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Reporting guidelines for Gel Electrophoresis experiments:
Guidelines for reporting the use of gel electrophoresis in proteomics, Nature Biotechnology 26, 863 (2008) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Reporting guidelines for Gel Informatics experiments:
Guidelines for reporting the use of gel image informatics in proteomics, Nature Biotechnology 28, 655-656 (2010) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Reporting guidelines for Column Chromatography experiments:
Guidelines for reporting the use of column chromatography in proteomics, Nature Biotechnology 28, 654 (2010) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Reporting guidelines for Capillary Electrophoresis experiments:
Guidelines for reporting the use of capillary electrophoresis in proteomics, Nature Biotechnology 28, 654-655 (2010) -- DOI -- PubMed.
(Free PDF/HTML download direct from Nature Biotechnology.)

Up-to-date released documents and working drafts available from this site here

MIAPE is now registered with the MIBBI Project (Minimum Information for Biological and Biomedical Investigations), which promotes and coordinates the development and management of Minimum Information (MI) specifications from across the biological and biomedical sciences.

Implementers

ProteoRed: see bioinformatics team wiki page.

 Chris Taylor, chris.taylor[@]ebi.ac.uk, September 25th, 2007. Modified by Andy Jones, Nov 2009. Modified by Salvador Martínez-Bartolomé, Apr 2011